The main objectives of this study were to elucidate the effect of multi-enzyme and/or probiotics supplementation on performance, antioxidant status, some blood biochemical parameters and immune responses of broiler chickens. A total of 120, unsexed day-old, broiler chicks were randomly assigned into four groups of 30 chicks in five replicates, six birds each. The first group was used as control and fed the basal diet while, chicks in the 2nd,3rd and 4th groups were fed the basal diet supplemented with multi enzymes (Avizyme at 0.20 g/kg diet), probiotics (Biacton at 0.50 g/kg diet) either singly or in combination. Results showed that live body weight and body weight gain were significantly improved by dietary treatments compared to control group. All treatments had significantly increased dressing and total edible parts (%) and decreased abdominal fat, with the best results being achieved by the combined multi-enzyme and probiotic supplementation. Blood constituents (plasma proteins and lipid fractions, thyroid hormones, especially T3 and glucose) were significantly affected by treatments. There are significant improvements in hematological traits including RBCs and WBCs counts, hemoglobin concentration, PCV (%), MCV, MCH and lymphocytes (%). Antioxidant status indices and enzymes including TAC, GSH, and SOD did not significantly affected by different treatments, however, glutathione peroxidase (GPX) activity was significantly higher in broilers fed the supplemented diets compared to the control one. Immune responses in terms of the main lymphoid organs weight (Bursa and Thymus), immunoglobulins (α–globulin, β–globulin, γ-globulin, IgA, and IgG), Phagocytic activity (PA), Phagocytic index (PI), lysozyme activity (LA), Bactericidal activity (BA) and Lymphocyte transformation test (LTT) were significantly improved by treatments compared with the control. It is concluded that a mixture of 0.2g of multi-enzyme plus 0.5g of probiotics /kg diet could be used to improve growth traits and enhance immunity of broiler chicks.