A total of 24 primiparous rabbit does of California (CAL) breed in three different reproductive statuses were used to assess the profile of some physiological, biochemical and hormonal parameters. The 3 reproductive statuses were before mating, during pregnancy and after parturition. Feed and water were provided ad libitum. Does body weights were weighed weekly. Rectal temperatures (RT), respiratory rate (RR) and pulse rate (PR) of does were recorded every other day. Serum total protein, albumin, cholesterol, glucose, urea and creatinine concentrations were determined twice a week. Growth hormone (GH), Estrogen (E₂), Progesterone (P₄), and Triiodotheronine (T₃) were determined in rabbit does within each of the 3 physiological statues. Body weight increased in pregnant rabbits than those before pregnancy and after parturition. No significant variations were found among biochemical parameters during these 3 physiological conditions except for albumin value that was lower after parturition in comparison to the other 2 status. No significant differences were observed in hormone values among rabbit groups except for progesterone that was higher during pregnancy in comparison to the other 2 status. It  could be concluded that rabbit does adjust their internal homeostasis during pregnancy and suckling. In addition, there is a positive correlation between some of the studied parameters indicating healthy status of the experimental does.