Culex pipiens is a major vector transmitting a variety of arthropod-borne pathogens. Cx pipiens were collected then identified morphologically and by mitochondrial cytochrome oxidase I gene for accurate species confirmation. Mosquito's' gut plays an important role in infection susceptibility. A baseline proteomic dataset is needed to give insights into the physiology of blood-feeding and to understand functional proteins in mosquito behavior following a blood meal ultimately leading to establishing effective control strategies. As the changes in protein expression of Cx. pipiens gut after sugar and blood-feeding has not been investigated, a proteomic analysis of gut tissue was carried out using Nano LC/mass spectrometry (LC-MS) and offline matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-MS. There were 56 functional putative proteins in the gut of sugar-fed Cx. pipiens females and 37 proteins gut of blood-fed Cx. pipiens females with molecular weight ranging from 800.4 to 2969.4 Da and from 818.3 to 3207.6 Da for sugar and blood-fed gut females, respectively. 39 and 20 proteins were uniquely identified in sugar-fed and blood-fed, respectively, while 17 proteins were common by the two biological groups. Data are available via ProteomeXchange with identifier PXD030533.