Six new Schiff bases were synthesized via reaction of benzidine, [1,1'-biphenyl]-4,4'-diyldimethanamine, 3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diamine, 2,2'-dimethyl-[1,1'-biphenyl]-4,4'-diamine, ethane-1,2-diamine and propane-1,3-diamine with 5,5-dimethylcyclohexane-1,3-dione in acidic medium of acetic acid. The structures of new compounds were elucidated with NMR, FTIR, UV and elemental analysis and antimicrobial activity of them were applied. Three different methods were used Conventional, microwave and grinding methods, and concluded that the grinding and clicking method is the best in terms of the quantity of the product and the time required for the reaction to be very fast, As well as protecting the health of workers and the environment.