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123148

Efficacy of Some Pyrethroid Nanoemulsions against cotton Leafworm Spodoptera littoralis (Boisd.): Toxicity, Biochemical and Molecular Docking Studies

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Nano chemistry

Abstract

Although pesticides are favorable for controlling the pests because more than 30– 40 % of the Although pesticides are favorable for pest controlling because more than 30– 40 % of the food production is lost due to pests, however, the extensive use of pesticides has harmful effects on the environment and health. Recently, nanotechnology techniques are used in developing agrochemical formulations, nanoemulsions as new approach have attracted great attention in delivery of many poorly soluble in water active compounds. In this study some most widely used pyrethroid insecticides (cypermethrin, deltamethrin and lambdacyhalothrin) were formulated as nanoemulsions by high energy method (ultrasonication). The droplet sizes of prepared nanoemulsions have been investigated by Dynamic Light Scattering (DLS) and Transmission Electron Microscope (TEM), characterization result reveals the size of particles in the range 24.42-84.99 nm. Formulated nanoemulsions in comparative with the active ingredient (ai) and emulsifier concentrate (EC) were evaluated against 4th instar larvae of S. littoralis, and the in vivo activity levels of targeted enzymes, acetylcholinesterase (AChE) and adenosine triphosphate (ATPase) were studied in two bioassay methods (leaf dipping and topical application). Our finding showed that cypermethrin nanoemulsion has the highest activity with LC50 = 19.92 mg/l and LD50= 2.11 ng/lavra. Furthermore, the developed nano-formulation showed good toxicity levels on AChE and ATPase compared to the active ingredient and EC formulations. The protein-ligands docking were also studied and the docking poses showed that the insecticides had a good binding affinity to the active site of the target enzymes. On this basis, these results suggest that oil in water (O/W) nanoemulsions would be very useful and can be applied for delivery insecticide formulations.

DOI

10.21608/ejchem.2020.45275.2946

Keywords

Nanoemulsion, Pyrethroid insecticides, Spodoptera littoralis, Biochemical studies, docking

Authors

First Name

Amany

Last Name

Abd Elnabi

MiddleName

Desoky

Affiliation

Researcher at Plant Protection Research Institute, Agricultural Research Center, Egypt

Email

amany.desoky@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Badawy

MiddleName

E.I.

Affiliation

Department of Pesticide Chemistry and Technology, Faculty of Agriculture, 21545-El-Shatby, Alexandria University, Alexandria, Egypt

Email

meltaher@yahoo.com

City

Alexandria

Orcid

-

First Name

Abdel-Fattah

Last Name

Saad

MiddleName

S. A.

Affiliation

Plant Protection Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria, Egypt

Email

abdelfattah.saad99@gmail.com

City

alexandria

Orcid

-

First Name

Sondos

Last Name

Mohamed

MiddleName

A.

Affiliation

Agriculture Research Center, Plant Protection Research Institute (PPRI). Egypt

Email

sondos_m2000@yahoo.com

City

cairo

Orcid

-

Volume

64

Article Issue

2

Related Issue

21013

Issue Date

2021-02-01

Receive Date

2020-10-13

Publish Date

2021-02-01

Page Start

1,047

Page End

1,055

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_123148.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=123148

Order

46

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Efficacy of Some Pyrethroid Nanoemulsions against cotton Leafworm Spodoptera littoralis (Boisd.): Toxicity, Biochemical and Molecular Docking Studies

Details

Type

Article

Created At

22 Jan 2023