Background: The most common bacterial infection among children is Urinary Tract Infection (UTI). Early diagnosis and good treatment of UTI is very important as the risk of renal damage is increased in children below the age of five years which result of morbidity. The aim of this study was to estimate the prevalence of urinary tract infection (UTI) in children attending Pediatric outpatient clinic in Zagazig University Children's Hospital. Also to determine related risk factors, isolate the organisms that cause UTI in children and antibiotics susceptibility patterns. Methods: This Cross sectional descriptive study, was conducted on 600 children, (377 males and 223 females) from two to seven years old attending to pediatric outpatient clinic ZUH , All patient groups were exposed to full medical history, physical examination, Dipstick analysis by using both nitrite and leukocyte esterase detector, Microscopic examinations and urine culture for positive cases. Results: The prevalence of UTI between children included in the current study was (7%). LE positive were 56 (9.3%), Nitrite positive were 47 (7.8%) and both LE and Nitrite positive were 17 (2.8%). Conclusion: The prevalence of UTI was 7 % in our study, E - Coli was detected to be the most common organism, Cefotaxime and Amikacin were detected to be the most common antibiotic sensitive to the isolates.