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USING DIAZOTROPHIC ENDOPHYTES IN IMPROVING SOME CEREAL PRODUCTION UNDER SALINE DESERT CONDITION

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Soil

Abstract

The present study was conducted to develop the bacterial consortia of salt tolerant endophytic diazotrohs. Forty three endophytic bacterial isolates were assayed for their nitrogen fixation and hormone production at different NaCl concentrations. All selected endophytic Azotobacter and Azospirillum isolates exhibited nitrogenase activities  ranged from 15.4 to 96.1 n.mole C2H4 /ml/h, indol acetic acid ranged from 31 to 168 μg/ml and gibberellins from 2.8 to 29.4 μg/ml at NaCl ranged from 1 to 5%.  Vital staining technique of wheat and barley seedlings revealed the presence of pink to purple stained endophytic bacteria in their root tissues. Different bacterial consortia were developed from the highly efficient endophytic nitrogen fixers. Two highly efficient bacterial endophytic consortia at 1% NaCl were selected for further lab and field experiments. The two bacterial consortia were investigated for their nitrogen fixation activities and indole acetic acid (IAA) production under different concentrations of salt. The obtained results revealed a dramatic decrease in the amount of fixed N with increasing the salt content from 1 to 10% NaCl for both consortia. The consortium of barley endophytes exhibited the highest IAA production at 5% NaCl, while that of wheat exhibited the maximum IAA at 1% NaCl.
                        Two field experiments conducted at Ras Sudr Experimental Station showed that inoculation of wheat and barley seeds with endophytic bacterial consortia significantly increased all the growth parameters, nitrogen content and uptake of two plants under salinity stress. Also, bacterial consortium of wheat endophytes along with half dose of nitrogen achieved the highest significant wheat and barley yields. Inoculation with endophytic bacterial consortia had a positive effect on the count of bacterial nitrogen fixers and dehydrogenase in the rhizoshere.   

DOI

10.21608/ejdr.2017.6499

Keywords

Endophytes, consortia, salt stress, Wheat, Barley

Authors

First Name

Amal

Last Name

Omer

MiddleName

M.

Affiliation

Department of Soil Fertility and Microbiology, Desert Research Center, El-Matareya, Cairo, Egypt

Email

amal_omaram@yahoo.com

City

-

Orcid

-

Volume

67

Article Issue

1

Related Issue

1031

Issue Date

2017-06-01

Receive Date

2017-05-03

Publish Date

2017-07-01

Page Start

210

Page End

229

Print ISSN

1687-8043

Online ISSN

2356-9875

Link

https://ejdr.journals.ekb.eg/article_6499.html

Detail API

https://ejdr.journals.ekb.eg/service?article_code=6499

Order

12

Type

Original Article

Type Code

117

Publication Type

Journal

Publication Title

Egyptian Journal of Desert Research

Publication Link

https://ejdr.journals.ekb.eg/

MainTitle

USING DIAZOTROPHIC ENDOPHYTES IN IMPROVING SOME CEREAL PRODUCTION UNDER SALINE DESERT CONDITION

Details

Type

Article

Created At

22 Jan 2023