The present study was conducted to investigate the harmful effects of daily exposure to microwave radiation on the cornea of rats. Forty eight healthy mature male Wister Albino rats (180-200 g) were classified into 6 groups of 8 rats each. Group (I) used as control group which was sham exposed. Groups (II, III, IV, V and VI) were exposed to 1.944×〖10〗^(-2)mW microwave radiation 8 hours a day for one, three, five, seven and nine days respectively. All rats were decapitated and rats 'eyes were enucleated immediately at the end of exposure period. Total soluble protein content, FTIR analysis and histopathological examination were carried out on cornea. The results we obtained showed that ,total soluble protein content of cornea decreased by increasing exposure time to microwave radiation .The most prominent changes in FTIR was detected in NHOH ,CH stretching and finger print regions after irradiation with microwave for successive 9 days (8 hours/day). Histopathological examination of cornea revealed that; rats exposed to 1.944×〖10〗^(-2) mW microwave radiation for 1 day and 3 days showing no change in all corneal layers. The pronounced change was observed after 5, 7 and 9 days of daily exposure to microwave radiation. Finally, it is concluded that daily exposure to microwave radiation has the potential to cause biological effects in corneal tissue.