This research aimed at measuring the quality of extension organization and the quality of agricultural extension service from the opinions of extension personnel in Assiut Governorate, and to identify the effect of the quality of extension organization's dimensions on the quality of agricultural extension service. Data were collected using questionnaire from 73 extension personnel in Assiut Governorate. Frequencies, percentages, arithmetic mean, and multiple regression analysis were used for analyzing data. The results showed a decline in the respondents' perceptions in contrast to their expectations of the quality of extension organization at all dimensions. It also came out that there is a negative gap between the respondents' perceptions and expectations of the agricultural extension service quality. Multiple regression analysis showed a significant effect of all dimensions of the quality of extension organization on the quality of agricultural extension service, and that these dimensions explain 62% of the variance in agricultural extension service quality.