Beta
ManageSearchChatDiscover
2523

Interaction of Methomyl with Brown Garden Snail, Eobania vermiculata (Müller), and White Snail, Theba pisana (Müller) Acetylcholinesterase Activity

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Comparative toxicity of methomyl against two land snails Eobania vermiculata and Theba pisana was investigated. The Michaelis constant(Km) and maximal velocity(Vmax) valuesof E. vermiculata and T. pisana AChE were compared. Finally, the in vivo and in vitro inhibition of E. vermiculata and T. pisana acetylcholinesterase by methomyl was studied. Results revealed that, the median lethal concentrations (LC50s values) of methomyl against T. pisana were 58.85, 18.71 and 3.87 % after 24, 48 and 72hrs post-treatment,respectively. On the other hand, LC50s values of methomyl against E. vermiculata were 2.94, 0.92 and 0.38 % after the same time, respectively. The Km values were 0.098 and 0.042 mM and Vmax values were 0.042 and 0.052 mmole/min for E. vermiculata and T. pisana AChE, respectively. Specific activity of T. pisana AChE is 1.43 times the E. vermiculata AChE specific activity. When T. pisana was exposed to methomyl at concentrations of 375, 750 and 1875 ppm the inhibition percentages of AChE were 88.74, 89.44 and 92.25%, respectively. On the other hand, methomyl revealed inhibition percentages of 42.3, 51.4 and 75.3% of E. vermiculata AChE activity at concentrations of 37.6, 75.2 and 188.0 ppm, respectively. The in vitro studies showed that, the sensitivity of T. pisana AChE (I50 = 1.3 µM) is 4.1 times more than the sensitivity of E. vermiculata AChE (I50 = 5.3 µM) to methomyl.  

DOI

10.21608/asejaiqjsae.2014.2523

Authors

First Name

Eshra

Last Name

E.H

MiddleName

-

Affiliation

Plant Protection Research Institute, Agricultural Research Center, Egypt.

Email

eheshra@yahoo.com

City

-

Orcid

-

First Name

Shimaa

Last Name

S. A

MiddleName

-

Affiliation

Central Agricultural Pesticide LaboratoryAgricultural Research Center, Egypt.

Email

-

City

-

Orcid

-

First Name

M.S

Last Name

EL- Shahaat

MiddleName

-

Affiliation

Plant Protection Research Institute, Agricultural Research Center, Egypt.

Email

-

City

-

Orcid

-

Volume

35

Article Issue

January-March

Related Issue

375

Issue Date

2014-01-01

Receive Date

2014-02-06

Publish Date

2014-03-01

Page Start

16

Page End

22

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 35_Issue January-March_Pages 16-22.pdf

PDF . 210.6kB

Link

https://asejaiqjsae.journals.ekb.eg/article_2523.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=2523

Order

3

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Interaction of Methomyl with Brown Garden Snail, Eobania vermiculata (Müller), and White Snail, Theba pisana (Müller) Acetylcholinesterase Activity

Details

Type

Article

Created At

22 Jan 2023